Plasmid Preparation:Article Title: HIF-2α dependent lipid storage promotes endoplasmic reticulum homeostasis in clear cell renal cell carcinoma
Article Snippet: Bortezomib was purchased from Cell Signaling Technologies (Cat 2204S). .. The lentiviral vector PLKO.1 SCR (Plasmid No. 17920) was obtained from Addgene. pLKO.1 vectors expressing shHIF1A_9 (TRCN0000003809), shHIF2A_6 (TRCN0000003806), shHIFA_7 (TRCN0000003807), shPLIN2_1 (TRCN0000136605), shPLIN2_2 (TRCN0000136481), shPPARG_2 (TRCN0000001672), and shPPARG_3 (TRCN0000001673) were obtained from the Broad Institute TRC shRNA library. .. The GIPZ vector expressing shHIF1A_52 (V3LMM_441752) was obtained from Dharmacon.
Article Title: Glycogen metabolism is dispensable for tumor progression in clear cell renal cell carcinoma
Article Snippet: .. For shRNAs, the lentiviral vector pLKO.1 SCR (scrambled shRNA plasmid no. 17920) was from Addgene. shRNAs targeting HIF1A (shHIF1A_52, shHIF1A_9) and EPAS1 (shHIF2A_6, shHIF2A_7) were described previously . .. For genetic knockout using CRISPR/Cas9, the lentiviral vector LentiCRISPR v2 (plasmid no. 52961) and LentiCRISPRv2GFP (plasmid no. 82416) were obtained from Addgene.
Article Title: HIF-2α dependent lipid storage promotes endoplasmic reticulum homeostasis in clear cell renal cell carcinoma
Article Snippet: Bortezomib was purchased from Cell Signaling Technologies (Cat 2204S). .. Plasmids, lentivirus production, and viral transduction The lentiviral vector PLKO.1 SCR (Plasmid No. 17920) was obtained from Addgene. pLKO.1 vectors expressing shHIF1A_9 (TRCN0000003809), shHIF2A_6 (TRCN0000003806), shHIFA_7 (TRCN0000003807), shPLIN2_1 (TRCN0000136605), shPLIN2_2 (TRCN0000136481), shPPARG_2 (TRCN0000001672), and shPPARG_3 (TRCN0000001673) were obtained from the Broad Institute TRC shRNA library. .. The GIPZ vector expressing shHIF1A_52 (V3LMM_441752) was obtained from Dharmacon.
Article Title: HIF2α-Dependent Lipid Storage Promotes Endoplasmic Reticulum Homeostasis in Clear-Cell Renal Cell Carcinoma
Article Snippet: .. © 2015 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from 16 Plasmids, lentivirus production, and viral transduction The lentiviral vector PLKO.1 SCR (Plasmid No. 17920) was obtained from Addgene. pLKO.1 vectors expressing shHIF1A_9 (TRCN0000003809), shHIF2A_6 (TRCN0000003806), shHIFA_7 (TRCN0000003807), shPLIN2_1 (TRCN0000136605), shPLIN2_2 (TRCN0000136481), shPPARG_2 (TRCN0000001672), and shPPARG_3 (TRCN0000001673) were obtained from the Broad Institute TRC shRNA library. .. The GIPZ vector expressing shHIF1A_52 (V3LMM_441752) was obtained from Dharmacon.
Expressing:Article Title: HIF-2α dependent lipid storage promotes endoplasmic reticulum homeostasis in clear cell renal cell carcinoma
Article Snippet: Bortezomib was purchased from Cell Signaling Technologies (Cat 2204S). .. The lentiviral vector PLKO.1 SCR (Plasmid No. 17920) was obtained from Addgene. pLKO.1 vectors expressing shHIF1A_9 (TRCN0000003809), shHIF2A_6 (TRCN0000003806), shHIFA_7 (TRCN0000003807), shPLIN2_1 (TRCN0000136605), shPLIN2_2 (TRCN0000136481), shPPARG_2 (TRCN0000001672), and shPPARG_3 (TRCN0000001673) were obtained from the Broad Institute TRC shRNA library. .. The GIPZ vector expressing shHIF1A_52 (V3LMM_441752) was obtained from Dharmacon.
Article Title: HIF-2α dependent lipid storage promotes endoplasmic reticulum homeostasis in clear cell renal cell carcinoma
Article Snippet: Bortezomib was purchased from Cell Signaling Technologies (Cat 2204S). .. Plasmids, lentivirus production, and viral transduction The lentiviral vector PLKO.1 SCR (Plasmid No. 17920) was obtained from Addgene. pLKO.1 vectors expressing shHIF1A_9 (TRCN0000003809), shHIF2A_6 (TRCN0000003806), shHIFA_7 (TRCN0000003807), shPLIN2_1 (TRCN0000136605), shPLIN2_2 (TRCN0000136481), shPPARG_2 (TRCN0000001672), and shPPARG_3 (TRCN0000001673) were obtained from the Broad Institute TRC shRNA library. .. The GIPZ vector expressing shHIF1A_52 (V3LMM_441752) was obtained from Dharmacon.
Article Title: HIF2α-Dependent Lipid Storage Promotes Endoplasmic Reticulum Homeostasis in Clear-Cell Renal Cell Carcinoma
Article Snippet: .. © 2015 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from 16 Plasmids, lentivirus production, and viral transduction The lentiviral vector PLKO.1 SCR (Plasmid No. 17920) was obtained from Addgene. pLKO.1 vectors expressing shHIF1A_9 (TRCN0000003809), shHIF2A_6 (TRCN0000003806), shHIFA_7 (TRCN0000003807), shPLIN2_1 (TRCN0000136605), shPLIN2_2 (TRCN0000136481), shPPARG_2 (TRCN0000001672), and shPPARG_3 (TRCN0000001673) were obtained from the Broad Institute TRC shRNA library. .. The GIPZ vector expressing shHIF1A_52 (V3LMM_441752) was obtained from Dharmacon.
shRNA:Article Title: HIF-2α dependent lipid storage promotes endoplasmic reticulum homeostasis in clear cell renal cell carcinoma
Article Snippet: Bortezomib was purchased from Cell Signaling Technologies (Cat 2204S). .. The lentiviral vector PLKO.1 SCR (Plasmid No. 17920) was obtained from Addgene. pLKO.1 vectors expressing shHIF1A_9 (TRCN0000003809), shHIF2A_6 (TRCN0000003806), shHIFA_7 (TRCN0000003807), shPLIN2_1 (TRCN0000136605), shPLIN2_2 (TRCN0000136481), shPPARG_2 (TRCN0000001672), and shPPARG_3 (TRCN0000001673) were obtained from the Broad Institute TRC shRNA library. .. The GIPZ vector expressing shHIF1A_52 (V3LMM_441752) was obtained from Dharmacon.
Article Title: Glycogen metabolism is dispensable for tumor progression in clear cell renal cell carcinoma
Article Snippet: .. For shRNAs, the lentiviral vector pLKO.1 SCR (scrambled shRNA plasmid no. 17920) was from Addgene. shRNAs targeting HIF1A (shHIF1A_52, shHIF1A_9) and EPAS1 (shHIF2A_6, shHIF2A_7) were described previously . .. For genetic knockout using CRISPR/Cas9, the lentiviral vector LentiCRISPR v2 (plasmid no. 52961) and LentiCRISPRv2GFP (plasmid no. 82416) were obtained from Addgene.
Article Title: HIF-2α dependent lipid storage promotes endoplasmic reticulum homeostasis in clear cell renal cell carcinoma
Article Snippet: Bortezomib was purchased from Cell Signaling Technologies (Cat 2204S). .. Plasmids, lentivirus production, and viral transduction The lentiviral vector PLKO.1 SCR (Plasmid No. 17920) was obtained from Addgene. pLKO.1 vectors expressing shHIF1A_9 (TRCN0000003809), shHIF2A_6 (TRCN0000003806), shHIFA_7 (TRCN0000003807), shPLIN2_1 (TRCN0000136605), shPLIN2_2 (TRCN0000136481), shPPARG_2 (TRCN0000001672), and shPPARG_3 (TRCN0000001673) were obtained from the Broad Institute TRC shRNA library. .. The GIPZ vector expressing shHIF1A_52 (V3LMM_441752) was obtained from Dharmacon.
Article Title: HIF2α-Dependent Lipid Storage Promotes Endoplasmic Reticulum Homeostasis in Clear-Cell Renal Cell Carcinoma
Article Snippet: .. © 2015 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from 16 Plasmids, lentivirus production, and viral transduction The lentiviral vector PLKO.1 SCR (Plasmid No. 17920) was obtained from Addgene. pLKO.1 vectors expressing shHIF1A_9 (TRCN0000003809), shHIF2A_6 (TRCN0000003806), shHIFA_7 (TRCN0000003807), shPLIN2_1 (TRCN0000136605), shPLIN2_2 (TRCN0000136481), shPPARG_2 (TRCN0000001672), and shPPARG_3 (TRCN0000001673) were obtained from the Broad Institute TRC shRNA library. .. The GIPZ vector expressing shHIF1A_52 (V3LMM_441752) was obtained from Dharmacon.
Transduction:Article Title: HIF-2α dependent lipid storage promotes endoplasmic reticulum homeostasis in clear cell renal cell carcinoma
Article Snippet: Bortezomib was purchased from Cell Signaling Technologies (Cat 2204S). .. Plasmids, lentivirus production, and viral transduction The lentiviral vector PLKO.1 SCR (Plasmid No. 17920) was obtained from Addgene. pLKO.1 vectors expressing shHIF1A_9 (TRCN0000003809), shHIF2A_6 (TRCN0000003806), shHIFA_7 (TRCN0000003807), shPLIN2_1 (TRCN0000136605), shPLIN2_2 (TRCN0000136481), shPPARG_2 (TRCN0000001672), and shPPARG_3 (TRCN0000001673) were obtained from the Broad Institute TRC shRNA library. .. The GIPZ vector expressing shHIF1A_52 (V3LMM_441752) was obtained from Dharmacon.
Article Title: HIF2α-Dependent Lipid Storage Promotes Endoplasmic Reticulum Homeostasis in Clear-Cell Renal Cell Carcinoma
Article Snippet: .. © 2015 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from 16 Plasmids, lentivirus production, and viral transduction The lentiviral vector PLKO.1 SCR (Plasmid No. 17920) was obtained from Addgene. pLKO.1 vectors expressing shHIF1A_9 (TRCN0000003809), shHIF2A_6 (TRCN0000003806), shHIFA_7 (TRCN0000003807), shPLIN2_1 (TRCN0000136605), shPLIN2_2 (TRCN0000136481), shPPARG_2 (TRCN0000001672), and shPPARG_3 (TRCN0000001673) were obtained from the Broad Institute TRC shRNA library. .. The GIPZ vector expressing shHIF1A_52 (V3LMM_441752) was obtained from Dharmacon.
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